ADDITIVE MANUFACTURING DALAM REKAYASA JARINGAN TULANG: Desain dan Manufaktur Bone Scaffold
Kata Kunci:
ADDITIVE MANUFACTURING, JARINGAN TULANG, BONE SCAFFOLDSinopsis
Perkembangan teknologi pencetakan tiga dimensi (3D printing) dalam beberapa dekade terakhir telah membuka peluang baru dalam bidang rekayasa biomedis. Salah satu teknologi yang banyak digunakan adalah Fused Deposition Modeling (FDM), yang memungkinkan pembuatan struktur bone scaffold dengan desain yang kompleks, porositas yang terkontrol, serta kemampuan kustomisasi sesuai kebutuhan klinis. Melalui teknologi ini, pengembangan bone scaffold untuk mendukung regenerasi jaringan tulang dapat dilakukan secara lebih efisien dan presisi.
Buku ini disusun berdasarkan kajian literatur dan hasil analisis ilmiah terkait penerapan teknologi FDM dalam proses manufaktur bone scaffold. Pembahasan dalam buku ini mencakup konsep dasar rekayasa jaringan tulang, karakteristik tulang manusia, prinsip teknologi additive manufacturing, desain arsitektur scaffold, parameter proses pencetakan FDM, serta material biomaterial yang umum digunakan dalam pembuatan scaffold tulang. Dengan demikian, buku ini diharapkan dapat memberikan gambaran yang komprehensif mengenai perkembangan teknologi FDM dalam bidang bone tissue engineering.
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KATA PENGANTAR
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DAFTAR ISI
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DAFTAR TABEL
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DAFTAR GAMBAR
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DAFTAR SINGKATAN
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01. PENDAHULUAN
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02. STRUKTUR DAN KARAKTERISTIK TULANG MANUSIA
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03. BONE TISSUE ENGINEERING DAN SCAFFOLD
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04. TEKNOLOGI ADDITIVE MANUFACTURING DALAM REKAYASA JARINGAN TULANG
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05. TEKNOLOGI FUSED DEPOSITION MODELING (FDM)
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06. DESAIN ARSITEKTUR BONE SCAFFOLD
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07. PARAMETER PROSES FDM PADA PEMBUATAN BONE SCAFFOLD
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08. MATERIAL UNTUK BONE SCAFFOLD BERBASIS FDM
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09. TANTANGAN DAN ARAH PENELITIAN KE DEPAN
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DAFTAR REFERENSI
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GLOSARIUM
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INDEKS
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PROFIL PENULIS
Unduhan
Referensi
Abdulhameed, O., Al-Ahmari, A., Ameen, W., & Mian, S. H. (2019). Additive manufacturing: Challenges, trends, and applications. Advances in Mechanical Engineering, 11(2), 1–27. https://doi.org/10.1177/1687814018822880
Ahmad, S. Z., Masood, M. H., Khattab, M. U., Ahmad, S. S., Zaidi, S. A. A., & Khan, S. Z. (2025). Recent Developments in Novel TPMS Lattice Materials: Design Optimization, Performance Control, and Applications in Biomimetic Scaffolds. Materials, 18(22), 5209. https://doi.org/10.3390/ma18225209
Akbar Teguh Prakoso, Ardiansyah Syahrom, Amir Arifin, & Hasan Basri. (2024). Mechanical Characterisation of 3D-Printed Porous PLA Scaffolds with Complex Microarchitectures for Bone Tissue Engineering Applications. Journal of Advanced Research in Applied Sciences and Engineering Technology, 47(1), 39–55. https://doi.org/10.37934/araset.47.1.3955
Alam, F., Verma, P., Walaa Mohammad, Teo, J., Varadarajan, K. M., & Kumar, S. (2021). Architected poly(lactic acid)/poly(e-caprolactone)/ halloysite nanotube composite scaffolds enabled by 3D printing for biomedical applications. In Journal of Materials Science (Vol. 56, pp. 14070–14083). Springer.
Alavi, M. S., Memarpour, S., Pazhohan‐Nezhad, H., Salimi Asl, A., Moghbeli, M., Shadmanfar, S., & Saburi, E. (2023). Applications of poly(lactic acid) in bone tissue engineering: A review article. Artificial Organs, 47(9), 1423–1430. https://doi.org/10.1111/aor.14612
Alcorta-Sevillano, N., Macías, I., Infante, A., & Rodríguez, C. I. (2020). Deciphering the Relevance of Bone ECM Signaling. Cells, 9(12), 2630. https://doi.org/10.3390/cells9122630
Alizadeh-Osgouei, M., Li, Y., Vahid, A., Ataee, A., & Wen, C. (2021a). High strength porous PLA gyroid scaffolds manufactured via fused deposition modeling for tissue-engineering applications. Smart Materials in Medicine, 2(November 2020), 15–25.
https://doi.org/10.1016/j.smaim.2020.10.003
Alizadeh-Osgouei, M., Li, Y., Vahid, A., Ataee, A., & Wen, C. (2021b). High strength porous PLA gyroid scaffolds manufactured via fused deposition modeling for tissue-engineering applications. Smart Materials in Medicine, 2(November 2020), 15–25.
https://doi.org/10.1016/j.smaim.2020.10.003
Arefin, A. M. E., Khatri, N. R., Kulkarni, N., & Egan, P. F. (2021). Polymer 3D Printing Review: Materials, Process, and Design Strategies for Medical Applications. Polymers, 13(9), 1499. https://doi.org/10.3390/polym13091499
Ariadna, G. P., Marc, R., Teresa, P., & Joaquim, C. (2016). Optimization of Poli(ϵ-caprolactone) Scaffolds Suitable for 3D Cancer Cell Culture. In Procedia CIRP (Vol. 49, pp. 61–66). https://doi.org/10.1016/j.procir.2015.07.031
Ariffin, M. K. A., Sukindar, N. A., Hang Tuah Bin Baharudin, B. T., Jaafar, C. N. A. B., & Ismail, M. I. S. Bin. (2019). The effect of process parameters in extruding scaffold design using synthetic biomaterials. International Journal of Modern Manufacturing Technologies, 11(3 Special Issue), 9–20.
Arjunan, A., Demetriou, M., Baroutaji, A., & Wang, C. (2020a). Mechanical performance of highly permeable laser melted Ti6Al4V bone scaffolds. Journal of the Mechanical Behavior of Biomedical Materials, 102, 103517. https://doi.org/10.1016/j.jmbbm.2019.103517
Arjunan, A., Demetriou, M., Baroutaji, A., & Wang, C. (2020b). Mechanical performance of highly permeable laser melted Ti6Al4V bone scaffolds. Journal of the Mechanical Behavior of Biomedical Materials, 102(October 2019). https://doi.org/10.1016/j.jmbbm.2019.103517
Ashok, V., Ganesh, M. K., Maiti, S., Nallaswamy, D., & Heboyan, A. (2025). Evaluation of 3D‐Printed Polylactic Acid as a Bone Substitute: An Animal Study in a Rat Model. Clinical and Experimental Dental Research, 11(4). https://doi.org/10.1002/cre2.70201
Augustine, R., Nikolopoulos, V. K., & Camci-Unal, G. (2023). Hydrogel-Impregnated Self-Oxygenating Electrospun Scaffolds for Bone Tissue Engineering. Bioengineering, 10(7), 854. https://doi.org/10.3390/bioengineering10070854
Auras, R., Harte, B., & Selke, S. (2004). An overview of polylactides as packaging materials. Macromolecular Bioscience, 4(9), 835–864. https://doi.org/10.1002/mabi.200400043
Azad, M. A., Olawuni, D., Kimbell, G., Badruddoza, A. Z. M., Hossain, Md. S., & Sultana, T. (2020). Polymers for Extrusion-Based 3D Printing of Pharmaceuticals: A Holistic Materials–Process Perspective. Pharmaceutics, 12(2), 124. https://doi.org/10.3390/pharmaceutics12020124
Backes, E. H., Fernandes, E. M., Diogo, G. S., Marques, C. F., Silva, T. H., Costa, L. C., Passador, F. R., Reis, R. L., & Pessan, L. A. (2021). Engineering 3 printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration. Materials Science and Engineering C, 122.
https://doi.org/10.1016/j.msec.2021.111928
Bagde, A. D., Kuthe, A. M., Nagdeve, S. R., Dahake, S. W., Sapkal, P. S., Daronde, S. B., Lande, N. H., & Sarode, B. D. (2019). Geometric Modeling and Finite Element Simulation for Architecture Design of 3D Printed Bio-ceramic Scaffold Used in Bone Tissue Engineering. Journal of the Indian Institute of Science, 99(3), 361–374. https://doi.org/10.1007/s41745-019-00120-0
Bagwan, J. K., Ahuja, B. B., Mulay, A. V., & Jawale, K. J. (2022a). Geometrical analysis of extrusion based (Additively Manufactured) 3D designed scaffold for bone tissue Engineering: A finite element approach. Materials Today: Proceedings, 50, 1465–1471. https://doi.org/10.1016/
j.matpr.2021.09.049
Bagwan, J. K., Ahuja, B. B., Mulay, A. V., & Jawale, K. J. (2022b). Geometrical analysis of extrusion based (Additively Manufactured) 3D designed scaffold for bone tissue Engineering: A finite element approach. Materials Today: Proceedings, 50, 1465–1471. https://doi.org/10.1016/
j.matpr.2021.09.049
Bahraminasab, M. (2020a). Challenges on optimization of 3D-printed bone scaffolds. BioMedical Engineering OnLine, 19(1), 69. https://doi.org/10.1186/s12938-020-00810-2
Bahraminasab, M. (2020b). Challenges on optimization of 3D printed bone scaffolds.
Bakhtiari, H., Nouri, A., & Tolouei-Rad, M. (2024). Fatigue Performance of 3D-Printed Poly-Lactic-Acid Bone Scaffolds with Triply Periodic Minimal Surface and Voronoi Pore Structures. Polymers, 16(15), 2145. https://doi.org/10.3390/polym16152145
Bandyopadhyay, A., Mitra, I., & Bose, S. (2020). 3D Printing for Bone Regeneration. Current Osteoporosis Reports, 18(5), 505–514. https://doi.org/10.1007/s11914-020-00606-2
Baumer, V., Isaacson, N., Kanakamedala, S., McGee, D., Kaze, I., & Prawel, D. (2024). Comparing ceramic Fischer-Koch-S and gyroid TPMS scaffolds for potential in bone tissue engineering. Frontiers in Bioengineering and Biotechnology, 12. https://doi.org/10.3389/fbioe.2024.1410837
Bello, S. A., Cruz-Lebrón, J., Rodríguez-Rivera, O. A., & Nicolau, E. (2023). Bioactive Scaffolds as a Promising Alternative for Enhancing Critical-Size Bone Defect Regeneration in the Craniomaxillofacial Region. ACS Applied Bio Materials, 6(11), 4465–4503. https://doi.org/10.1021/acsabm.3c00432
Bernardo, M. P., da Silva, B. C. R., & Mattoso, L. H. C. (2021). Development of three-dimensional printing filaments based on poly(lactic acid)/hydroxyapatite composites with potential for tissue engineering. Journal of Composite Materials, 55(17), 2289–2300. https://doi.org/10.1177/0021998320988568
Bikas, H., Stavropoulos, P., & Chryssolouris, G. (2016). Additive manufacturing methods and modeling approaches: A critical review. International Journal of Advanced Manufacturing Technology, 83(1–4), 389–405. https://doi.org/10.1007/
s00170-015-7576-2
Blatt, S., & Al‐Nawas, B. (2025). Fabrication of Bone for Guided Bone Regeneration: A Narrative Review of Current Principles, Clinical Applications, Challenges and Future Perspectives. Australian Dental Journal, 70(S1). https://doi.org/
10.1111/adj.70029
Cano-vicent, A., Tambuwala, M. M., Sarif, S., Barh, D., Alaa, A., Aljabali, A., Birkett, M., & Arjunan, A. (2021). Fused deposition modelling : Current status, methodology, applications and future prospects. Additive Manufacturing, 47(102378).
Cao, X., Sun, K., Luo, J., Chen, A., Wan, Q., Zhou, H., Zhou, H., Liu, Y., & Chen, X. (2025). Enhancing Osteogenesis and Mechanical Properties through Scaffold Design in 3D Printed Bone Substitutes. ACS Biomaterials Science & Engineering, 11(2), 710–729. https://doi.org/10.1021/acsbiomaterials.
4c01661
Carlier, E., Marquette, S., Peerboom, C., Denis, L., Benali, S., Raquez, J. M., Amighi, K., & Goole, J. (2019a). Investigation of the parameters used in fused deposition modeling of poly(lactic acid) to optimize 3D printing sessions. International Journal of Pharmaceutics, 565(May), 367–377. https://doi.org/10.1016/j.ijpharm.2019.05.008
Carlier, E., Marquette, S., Peerboom, C., Denis, L., Benali, S., Raquez, J. M., Amighi, K., & Goole, J. (2019b). Investigation of the parameters used in fused deposition modeling of poly(lactic acid) to optimize 3D printing sessions. International Journal of Pharmaceutics, 565(February), 367–377. https://doi.org/10.1016/j.ijpharm.2019.05.008
Chakraborty, R., Anoop, A. G., Thakur, A., Mohanta, G. C., & Kumar, P. (2023). Strategies To Modify the Surface and Bulk Properties of 3D-Printed Solid Scaffolds for Tissue Engineering Applications. ACS Omega, 8(6), 5139–5156. https://doi.org/10.1021/acsomega.2c05984
Charania, J., Lone, M., Randhir, N., Shete, R., & Khushale, K. (2023). Perception of Students and Faculty about Mounted Display of Dry Human Bones as Visual Educational Tool. Dev Sanskriti Interdisciplinary International Journal, 22, 12–16. https://doi.org/10.36018/dsiij.22.249
Charbe, N. B., Tambuwala, M., Palakurthi, S. S., Warokar, A., Hromić‐Jahjefendić, A., Bakshi, H., Zacconi, F., Mishra, V., Khadse, S., Aljabali, A. A., El‐Tanani, M., Serrano‐Aroca, Ã., & Palakurthi, S. (2023). Biomedical applications of three‐dimensional bioprinted craniofacial tissue engineering. Bioengineering & Translational Medicine, 8(1). https://doi.org/10.1002/btm2.10333
Chen, H., Han, Q., Wang, C., Liu, Y., Chen, B., & Wang, J. (2020). Porous Scaffold Design for Additive Manufacturing in Orthopedics: A Review. Frontiers in Bioengineering and Biotechnology, 8(June), 1–20. https://doi.org/10.3389/
fbioe.2020.00609
Chen, L., Al-Bayatee, S., Khurshid, Z., Shavandi, A., Brunton, P., & Ratnayake, J. (2021). Hydroxyapatite in oral care products—a review. Materials, 14(17). https://doi.org/10.3390/
ma14174865
Chen, Z., Liu, Y., Huang, J., Wang, H., Hao, M., Hu, X., Qian, X., Fan, J., Yang, H., & Yang, B. (2022). Enhanced In Vitro Biocompatible Polycaprolactone/Nano-Hydroxyapatite Scaffolds with Near-Field Direct-Writing Melt Electrospinning Technology. Journal of Functional Biomaterials, 13(4), 161. https://doi.org/10.3390/jfb13040161
Chinnasami, H., Dey, M. K., & Devireddy, R. (2023). Three-Dimensional Scaffolds for Bone Tissue Engineering. Bioengineering, 10(7), 759. https://doi.org/10.3390/
bioengineering10070759
Cho, Y. S., Gwak, S. J., & Cho, Y. S. (2021a). Fabrication of polycaprolactone/nano hydroxyapatite (Pcl/nha) 3d scaffold with enhanced in vitro cell response via design for additive manufacturing (dfam). Polymers, 13(9). https://doi.org/10.3390/polym13091394
Cho, Y. S., Gwak, S. J., & Cho, Y. S. (2021b). Fabrication of polycaprolactone/nano hydroxyapatite (Pcl/nha) 3d scaffold with enhanced in vitro cell response via design for additive manufacturing (dfam). Polymers, 13(9). https://doi.org/10.3390/polym13091394
Chocholata, P., Kulda, V., & Babuška, V. (2019). Fabrication of Scaffolds for Bone-Tissue Regeneration. Materials, 12(4), 568. https://doi.org/10.3390/ma12040568
Chouhan, G., & Bala Murali, G. (2024). Designs, advancements, and applications of three-dimensional printed gyroid structures: A review. Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering, 238(2), 965–987. https://doi.org/10.1177/
09544089231160030
Collins, M. N., Ren, G., Young, K., Pina, S., Reis, R. L., & Oliveira, J. M. (2021). Scaffold Fabrication Technologies and Structure/Function Properties in Bone Tissue Engineering. Advanced Functional Materials, 31(21). https://doi.org/10.1002/adfm.202010609
Daminabo, S. C., Goel, S., Grammatikos, S. A., Nezhad, H. Y., & Thakur, V. K. (2020). Fused deposition modeling-based additive manufacturing (3D printing): techniques for polymer material systems. Materials Today Chemistry, 16, 100248. https://doi.org/10.1016/j.mtchem.2020.100248
Daniel, A., & Suya Prem Anand, P. (2025). Advancements in SS 316L TPMS gyroid scaffold developed for bone tissue engineering. Surface Topography: Metrology and Properties, 13(2), 023001. https://doi.org/10.1088/2051-672X/add17a
de Misquita, M. R. D. O. F., Bentini, R., & Goncalves, F. (2016). The performance of bone tissue engineering scaffolds in in vivo animal models: A systematic review. Journal of Biomaterials Applications, 31(5), 625–636. https://doi.org/10.1177/08
85328216656476
Deomore, S. A., & Raykar, S. J. (2021a). Multi-criteria decision making paradigm for selection of best printing parameters of fused deposition modeling. Materials Today: Proceedings, 44, 2562–2565. https://doi.org/10.1016/j.matpr.2020.12.632
Deomore, S. A., & Raykar, S. J. (2021b). Multi-criteria decision making paradigm for selection of best printing parameters of fused deposition modeling. Materials Today: Proceedings, 44, 2562–2565. https://doi.org/10.1016/j.matpr.2020.12.632
Deshmukh, S., Chand, A., & Ghorpade, R. (2024). Bio-mechanical analysis of porous Ti-6Al-4V scaffold: a comprehensive review on unit cell structures in orthopaedic application. Biomedical Physics & Engineering Express, 10(6), 062003. https://doi.org/10.1088/2057-1976/ad8202
Dey, A., & Yodo, N. (2019). A systematic survey of FDM process parameter optimization and their influence on part characteristics. Journal of Manufacturing and Materials Processing, 3(3). https://doi.org/10.3390/jmmp3030064
Dias, D., Vale, A. C., Cunha, E. P. F., C. Paiva, M., Reis, R. L., Vaquette, C., & Alves, N. M. (2021). 3D-printed cryomilled poly(ε-caprolactone)/graphene composite scaffolds for bone tissue regeneration. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 109(7), 961–972. https://doi.org/10.1002/jbm.b.34761
Diez-Escudero, A., Andersson, B., Persson, C., & Hailer, N. P. (2021). Hexagonal pore geometry and the presence of hydroxyapatite enhance deposition of mineralized bone matrix on additively manufactured polylactic acid scaffolds. Materials Science and Engineering C, 125(December 2020). https://doi.org/10.1016/j.msec.2021.112091
Diez-Escudero, A., Harlin, H., Isaksson, P., & Persson, C. (2020). Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential. Journal of Tissue Engineering, 11. https://doi.org/10.1177/2041731420956541
Distler, T., Fournier, N., Grünewald, A., Polley, C., Seitz, H., Detsch, R., & Boccaccini, A. R. (2020). Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.00552
Domingos, M., Gloria, A., Coelho, J., Bartolo, P., & Ciurana, J. (2017). Three-dimensional printed bone scaffolds: The role of nano/micro-hydroxyapatite particles on the adhesion and differentiation of human mesenchymal stem cells. Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, 231(6), 555–564. https://doi.org/10.1177/0954411916680236
Doshi, M., Mahale, A., Kumar, S. S., & Deshmukh, S. (2021a). Printing parameters and materials affecting mechanical properties of FDM-3D printed Parts: Perspective and prospects. Materials Today: Proceedings, 50(5), 2269–2275. https://doi.org/10.1016/j.matpr.2021.10.003
Doshi, M., Mahale, A., Kumar, S. S., & Deshmukh, S. (2021b). Printing parameters and materials affecting mechanical properties of FDM-3D printed Parts: Perspective and prospects. Materials Today: Proceedings, 50(5), 2269–2275. https://doi.org/10.1016/j.matpr.2021.10.003
Du, T., Niu, Y., Jia, Z., Liu, Y., Qiao, A., Yang, H., & Niu, X. (2022). Orthophosphate and alkaline phosphatase induced the formation of apatite with different multilayered structures and mineralization balance. Nanoscale, 14(5), 1814–1825. https://doi.org/10.1039/D1NR06016C
Dubey, A., Vahabi, H., & Kumaravel, V. (2023). Antimicrobial and Biodegradable 3D Printed Scaffolds for Orthopedic Infections. ACS Biomaterials Science & Engineering, 9(7), 4020–4044. https://doi.org/10.1021/acsbiomaterials.3c00115
Dukle, A., Murugan, D., Nathanael, A., Rangasamy, L., & Oh, T.-H. (2022). Can 3D-Printed Bioactive Glasses Be the Future of Bone Tissue Engineering? Polymers, 14(8), 1627. https://doi.org/10.3390/polym14081627
Dwivedi, R., Kumar, S., Pandey, R., Mahajan, A., Nandana, D., Katti, D. S., & Mehrotra, D. (2020). Polycaprolactone as biomaterial for bone scaffolds: Review of literature. Journal of Oral Biology and Craniofacial Research, 10(1), 381–388. https://doi.org/10.1016/j.jobcr.2019.10.003
Dziaduszewska, M., & Zieliński, A. (2021). Structural and Material Determinants Influencing the Behavior of Porous Ti and Its Alloys Made by Additive Manufacturing Techniques for Biomedical Applications. Materials, 14(4), 712. https://doi.org/10.3390/ma14040712
Egan, P. F., Ferguson, S. J., & Shea, K. (2017). Design of Hierarchical Three-Dimensional Printed Scaffolds Considering Mechanical and Biological Factors for Bone Tissue Engineering. Journal of Mechanical Design, 139(6). https://doi.org/10.1115/1.4036396
Egan, P. F., Gonella, V. C., Engensperger, M., Ferguson, S. J., & Shea, K. (2017). Computationally designed lattices with tuned properties for tissue engineering using 3D printing. PLoS ONE, 12(8), 1–20. https://doi.org/10.1371/journal.pone.0182902
Elhattab, K., Hefzy, M. S., Hanf, Z., Crosby, B., Enders, A., Smiczek, T., Haghshenas, M., Jahadakbar, A., & Elahinia, M. (2021). Biomechanics of Additively Manufactured Metallic Scaffolds—A Review. Materials, 14(22), 6833. https://doi.org/10.3390/ma14226833
Esposito, C. C., Gervaso, F., Scalera, F., Padmanabhan, S. K., Madaghiele, M., Montagna, F., Sannino, A., Licciulli, A., & Maffezzoli, A. (2019). Highly loaded hydroxyapatite microsphere/ PLA porous scaffolds obtained by fused deposition modelling. Ceramics International, 45(2), 2803–2810. https://doi.org/10.1016/j.ceramint.2018.07.297
Fahmy, M. D., Jazayeri, H. E., Razavi, M., Masri, R., & Tayebi, L. (2016). Three‐Dimensional Bioprinting Materials with Potential Application in Preprosthetic Surgery. Journal of Prosthodontics, 25(4), 310–318. https://doi.org/10.1111/jopr.12431
Fan, D., Staufer, U., & Accardo, A. (2019). Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications. Bioengineering, 6(4), 113. https://doi.org/10.3390/bioengineering6040113
Feng, P., Liu, L., Yang, F., Min, R., Wu, P., & Shuai, C. (2025a). Shape/properties collaborative intelligent manufacturing of artificial bone scaffold: structural design and additive manufacturing process. Biofabrication, 17(1), 012005. https://doi.org/10.1088/1758-5090/ad905f
Feng, P., Liu, L., Yang, F., Min, R., Wu, P., & Shuai, C. (2025b). Shape/properties collaborative intelligent manufacturing of artificial bone scaffold: structural design and additive manufacturing process. Biofabrication, 17(1), 012005. https://doi.org/10.1088/1758-5090/ad905f
Fernandes, C., Moura, C., Ascenso, R. M. T., Amado, S., Alves, N., & Pascoal-Faria, P. (2016). Comprehensive Review on Full Bone Regeneration through 3D Printing Approaches. Intech, i(tourism), 13.
Ferri, J. M., Jordá, J., Montanes, N., Fenollar, O., & Balart, R. (2018). Manufacturing and characterization of poly(lactic acid) composites with hydroxyapatite. Journal of Thermoplastic Composite Materials, 31(7), 865–881. https://doi.org/10.1177/0892705717729014
Fiorillo, A. R., Forster, C. A., & Weishampel, D. B. (2023). Introduction to Dinosaurs: New Ideas from Old Bones papers honoring Peter Dodson. The Anatomical Record, 306(7), 1595–1601. https://doi.org/10.1002/ar.25241
Foroughi, A. H., Valeri, C., & Razavi, M. J. (2025). A review of computational optimization of bone scaffold architecture: methods, challenges, and perspectives. Progress in Biomedical Engineering, 7(1), 012003. https://doi.org/10.1088/2516-1091/ad879a
Forrestal, D. P., Klein, T. J., & Woodruff, M. A. (2017). Challenges in engineering large customized bone constructs. Biotechnology and Bioengineering, 114(6), 1129–1139. https://doi.org/10.1002/bit.26222
Foster, B. L. (2017). On the discovery of cementum. Journal of Periodontal Research, 52(4), 666–685. https://doi.org/10.1111/jre.12444
Franklin, D., & Marks, M. K. (2022). The professional practice of forensic anthropology: Contemporary developments and cross‐disciplinary applications. WIREs Forensic Science, 4(2). https://doi.org/10.1002/wfs2.1442
Gamsjaeger, S., Eriksen, E. F., & Paschalis, E. P. (2021). Effect of hormone replacement therapy on bone formation quality and mineralization regulation mechanisms in early postmenopausal women. Bone Reports, 14, 101055. https://doi.org/10.1016/j.bonr.2021.101055
Ghassemi, T., Shahroodi, A., Ebrahimzadeh, M. H., Mousavian, A., Movaffagh, J., & Moradi, A. (2018). Current concepts in scaffolding for bone tissue engineering. Archives of Bone and Joint Surgery, 6(2), 90–99. https://doi.org/10.22038/abjs.2018.26340.1713
Giannoudis, P. V., Dinopoulos, H., & Tsiridis, E. (2005). Bone substitutes: an update. Injury, 36 Suppl 3, 20–27. https://doi.org/10.1016/j.injury.2005.07.029
Gleadall, A., Visscher, D., Yang, J., Thomas, D., & Segal, J. (2018). Review of additive manufactured tissue engineering scaffolds: relationship between geometry and performance. Burns & Trauma, 6. https://doi.org/10.1186/s41038-018-0121-4
Gleeson, J., Plunkett, N., & O’Brien, F. (2010). Addition of hydroxyapatite improves stiffness, interconnectivity and osteogenic potential of a highly porous collagen-based scaffold for bone tissue regeneration. European Cells and Materials, 20, 218–230. https://doi.org/10.22203/eCM.v020a18
Grover, D., Kaur, N., & Kaur, G. (2021). Future dental device- 3-dimensional printing approach in dentistry. IP International Journal of Maxillofacial Imaging, 7(2), 37–47. https://doi.org/10.18231/j.ijmi.2021.009
Gu, L., Huang, R., Ni, N., Gu, P., & Fan, X. (2023). Advances and Prospects in Materials for Craniofacial Bone Reconstruction. ACS Biomaterials Science & Engineering, 9(8), 4462–4496. https://doi.org/10.1021/acsbiomaterials.3c00399
Guo, Y., Liang, K., & Ji, Y. (2019). New degradable composite elastomers of POC/PCL fabricated via in-situ copolymerization blending strategy. European Polymer Journal, 110(November 2018), 337–343. https://doi.org/10.1016/j.eurpolymj.2018.11.048
Hadjab, I., Farlay, D., Crozier, P., Douillard, T., Boivin, G., Chevalier, J., Meille, S., & Follet, H. (2021). Intrinsic properties of osteomalacia bone evaluated by nanoindentation and FTIRM analysis. Journal of Biomechanics, 117, 110247. https://doi.org/10.1016/j.jbiomech.2021.110247
Hammond, A. S. (2016). The Anthropoid Crista Trochanterica and the Hip Joint Capsule. The Anatomical Record, 299(1), 60–69. https://doi.org/10.1002/ar.23288
Haq, R. H. A., Rahman, M. N. A., Ariffin, A. M. T., Hassan, M. F., Yunos, M. Z., & Adzila, S. (2017). Characterization and Mechanical Analysis of PCL/PLA composites for FDM feedstock filament. IOP Conference Series: Materials Science and Engineering, 226(1). https://doi.org/10.1088/1757-899X/226/1/012038
Hayashi, T., Kanai, H., & Hayashi, T. (2001). Enzymatic Degradation of PCL fibers in vitro.pdf. In Polymer Journal (Vol. 33, Number 1, pp. 38–41). n.
He, S., Hu, Q., Sun, Y., Xu, Y., Huang, L., Cao, G., & Guo, T. (2023). Electrospun polycaprolactone incorporated with fluorapatite nanoparticles composite scaffolds enhance healing of experimental calvarial defect on rats. Annals of Translational Medicine, 11(9), 313–313. https://doi.org/10.21037/atm-22-4865
Heo, S. Y., Ko, S. C., Oh, G. W., Kim, N., Choi, I. W., Park, W. S., & Jung, W. K. (2019). Fabrication and characterization of the 3D-printed polycaprolactone/fish bone extract scaffolds for bone tissue regeneration. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 107(6), 1937–1944. https://doi.org/10.1002/jbm.b.34286
Hernandez, C. J. (2016). Cancellous bone. In Handbook of Biomaterial Properties, Second Edition (pp. 15–21). Springer New York. https://doi.org/10.1007/978-1-4939-3305-1_2
Hikmat, M., Rostam, S., & Ahmed, Y. M. (2021a). Investigation of tensile property-based Taguchi method of PLA parts fabricated by FDM 3D printing technology. Results in Engineering, 11, 100264. https://doi.org/10.1016/j.rineng.2021.100264
Hikmat, M., Rostam, S., & Ahmed, Y. M. (2021b). Investigation of tensile property-based Taguchi method of PLA parts fabricated by FDM 3D printing technology. Results in Engineering, 11, 100264. https://doi.org/10.1016/j.rineng.2021.100264
Hollister, S. J. (2006). Porous scaffold design for tissue engineering (vol 4, pg 518, 2005). Nature Materials, 5(7), 590.
Huang, D., Li, Z., Li, G., Zhou, F., Wang, G., Ren, X., & Su, J. (2025). Biomimetic structural design in 3D-printed scaffolds for bone tissue engineering. Materials Today Bio, 32, 101664. https://doi.org/10.1016/j.mtbio.2025.101664
Huang, S. H., Liu, P., Mokasdar, A., & Hou, L. (2013). Additive manufacturing and its societal impact: A literature review. International Journal of Advanced Manufacturing Technology, 67(5–8), 1191–1203. https://doi.org/10.1007/s00170-012-4558-5
Jaisingh, S. A., & Kumar, H. (2020). Fused Deposition modeling process parameters optimization and effect on mechanical properties and part quality: Review and reflection on present research. Materials Today: Proceedings, 21, 1659–1672. https://doi.org/10.1016/j.matpr.2019.11.296
Jalise, S. Z., Mehrabi, A., Habibi, S., Milan, P. B., & Rezapour, A. (2025). Three‐Dimensional Printing Applications for Bone Tissue Engineering: A Review. Polymers for Advanced Technologies, 36(4). https://doi.org/10.1002/pat.70152
Jammalamadaka, U., & Tappa, K. (2018). Recent Advances in Biomaterials for 3D Printing and Tissue Engineering. Journal of Functional Biomaterials, 9(1), 22. https://doi.org/10.3390/jfb9010022
Janmohammadi, M., Nourbakhsh, M. S., Bahraminasab, M., & Tayebi, L. (2023). Effect of Pore Characteristics and Alkali Treatment on the Physicochemical and Biological Properties of a 3D-Printed Polycaprolactone Bone Scaffold. ACS Omega, 8(8), 7378–7394. https://doi.org/10.1021/acsomega.2c05571
Jayaraman, P., Gandhimathi, C., Venugopal, J. R., Becker, D. L., Ramakrishna, S., & Srinivasan, D. K. (2015). Controlled release of drugs in electrosprayed nanoparticles for bone tissue engineering. Advanced Drug Delivery Reviews, 94, 77–95. https://doi.org/10.1016/j.addr.2015.09.007
Jayatissa, N. U., & Bhaduri, S. (2019). 3D Printed Polymer Scaffolds for Bone Tissue Regeneration. Journal of Emerging Investigators. https://doi.org/10.59720/18-059
Jiao, Z., Luo, B., Xiang, S., Ma, H., Yu, Y., & Yang, W. (2019). 3D printing of HA / PCL composite tissue engineering scaffolds. Advanced Industrial and Engineering Polymer Research, 2(4), 196–202. https://doi.org/10.1016/j.aiepr.2019.09.003
Joseph, A., & Uthirapathy, V. (2024). A Systematic Review of the Contribution of Additive Manufacturing toward Orthopedic Applications. ACS Omega, 9(44), 44042–44075. https://doi.org/10.1021/acsomega.4c04870
Kann, P. H., Pommerening, T., Brömer, R., John, C., & Hadji, P. (2025). High-Resolution Peripheral Quantitative Computed Tomography (HR-pQCT) as a new Method for Human Osteoarcheology. Osteologie, 34(04), 280–285. https://doi.org/10.1055/a-2703-6179
Kanwar, S., & Vijayavenkataraman, S. (2021). Design of 3D printed scaffolds for bone tissue engineering: A review. Bioprinting, 24(May), e00167. https://doi.org/10.1016/j.bprint.2021.e00167
Kao, C.-T., Chen, Y.-J., Huang, T.-H., Lin, Y.-H., Hsu, T.-T., & Ho, C.-C. (2020). Assessment of the Release Profile of Fibroblast Growth Factor-2-Load Mesoporous Calcium Silicate/Poly-ε-caprolactone 3D Scaffold for Regulate Bone Regeneration. Processes, 8(10), 1249. https://doi.org/10.3390/pr8101249
Karimi, M., Asadi‐Eydivand, M., Abolfathi, N., Chehrehsaz, Y., & Solati‐Hashjin, M. (2023). The effect of pore size and layout on mechanical and biological properties of 3D ‐printed bone scaffolds with gradient porosity. Polymer Composites, 44(2), 1343–1359. https://doi.org/10.1002/
pc.27174
Karimipour-Fard, P., Jeffrey, M. P., JonesTaggart, H., Pop-Iliev, R., & Rizvi, G. (2021a). Development, processing and characterization of Polycaprolactone/Nano-Hydroxyapatite/
Chitin-Nano-Whisker nanocomposite filaments for additive manufacturing of bone tissue scaffolds. Journal of the Mechanical Behavior of Biomedical Materials, 120. https://doi.org/10.1016/j.jmbbm.2021.104583
Karimipour-Fard, P., Jeffrey, M. P., JonesTaggart, H., Pop-Iliev, R., & Rizvi, G. (2021b). Development, processing and characterization of Polycaprolactone/Nano-Hydroxyapatite/Chitin-Nano-Whisker nanocomposite filaments for additive manufacturing of bone tissue scaffolds. Journal of the Mechanical Behavior of Biomedical Materials, 120. https://doi.org/10.1016/j.jmbbm.2021.104583
Karuppudaiyan, S., & Kingsly Jeba Singh, D. (2019). Design of scaffold with controlled internal architecture using fused deposition modeling (FDM). International Journal of Engineering and Advanced Technology, 9(1), 2764–2768. https://doi.org/10.35940/ijeat.A9769.109119
Karuppudaiyan, S., & Singh, D. K. J. (2019). Design of scaffold with controlled internal architecture using fused deposition modeling (FDM). International Journal of Engineering and Advanced Technology, 9(1), 2764–2768. https://doi.org/10.35940/ijeat.A9769.109119
Kaseem, M. (2019). Poly(Lactic Acid) Composites. Materials, 12(3586), 11–12. https://doi.org/doi:10.3390/ma12213586
Kelly, C. N., Miller, A. T., Hollister, S. J., Guldberg, R. E., & Gall, K. (2018). Design and Structure–Function Characterization of 3D Printed Synthetic Porous Biomaterials for Tissue Engineering. Advanced Healthcare Materials, 7(7). https://doi.org/10.1002/adhm.201701095
Khan, A. R., Gholap, A. D., Grewal, N. S., Jun, Z., Khalid, M., & Zhang, H.-J. (2025). Advances in smart hybrid scaffolds: A strategic approach for regenerative clinical applications. Engineered Regeneration, 6, 85–110. https://doi.org/10.1016/j.engreg.2025.02.002
Khan, A. R., Grewal, N. S., Jun, Z., Tawfiq, F. M. O., Tchier, F., Muhammad Zulqarnain, R., & Zhang, H.-J. (2024). Raising the Bar: Progress in 3D-Printed Hybrid Bone Scaffolds for Clinical Applications: A Review. Cell Transplantation, 33. https://doi.org/10.1177/09636897241273562
Khodaei, M., Amini, K., & Valanezhad, A. (2020). Fabrication and Characterization of Poly Lactic Acid Scaffolds by Fused Deposition Modeling for Bone Tissue Engineering. Journal Wuhan University of Technology, Materials Science Edition, 35(1), 248–251. https://doi.org/10.1007/s11595-020-2250-4
Khorasani, E., & Vahidi, B. (2025). 3D‐Printed Scaffolds for Cranial Bone Regeneration: A Systematic Review of Design, Materials, and Computational Optimization. Biotechnology and Bioengineering, 122(8), 1982–2008. https://doi.org/10.1002/bit.28994
Khosravani, M. R., & Reinicke, T. (2020a). Effects of raster layup and printing speed on strength of 3D-printed structural components. Procedia Structural Integrity, 28, 720–725. https://doi.org/10.1016/j.prostr.2020.10.083
Khosravani, M. R., & Reinicke, T. (2020b). Effects of raster layup and printing speed on strength of 3D-printed structural components. Procedia Structural Integrity, 28, 720–725. https://doi.org/10.1016/j.prostr.2020.10.083
Kim, C. G., Han, K. S., Lee, S., Kim, M. C., Kim, S. Y., & Nah, J. (2021). Fabrication of biocompatible polycaprolactone–hydroxyapatite composite filaments for the FDM 3D printing of bone scaffolds. Applied Sciences (Switzerland), 11(14). https://doi.org/10.3390/app11146351
Kim, K., Yeatts, A., Dean, D., & Fisher, J. P. (2010). Stereolithographic Bone Scaffold Design Parameters: Osteogenic Differentiation and Signal Expression. Tissue Engineering Part B: Reviews, 16(5), 523–539. https://doi.org/10.1089/ten.teb.2010.0171
Kim, T., See, C. W., Li, X., & Zhu, D. (2020). Orthopedic implants and devices for bone fractures and defects: Past, present and perspective. Engineered Regeneration, 1(April), 6–18. https://doi.org/10.1016/j.engreg.2020.05.003
Kolliopoulos, V., & Harley, B. A. (2024). Mineralized collagen scaffolds for regenerative engineering applications. Current Opinion in Biotechnology, 86, 103080. https://doi.org/10.1016/j.copbio.2024.103080
Kumar, A., Mir, M., Aldulijan, I., Mahajan, A., Anwar, A., Leon, C. H., Terracciano, A., Zhao, X., Su, T., Kalyon, D. M., Kumbar, S. G., & Yu, X. (2021). Load‐bearing biodegradable PCL‐PGA ‐beta TCP scaffolds for bone tissue regeneration. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 109(2), 193–200. https://doi.org/10.1002/jbm.b.34691
Kumar, M., & Sharma, V. (2021). Additive manufacturing techniques for the fabrication of tissue engineering scaffolds: a review. Rapid Prototyping Journal, 27(6), 1230–1272. https://doi.org/10.1108/RPJ-01-2021-0011
Kumar, R., Kumar, M., & Chohan, J. S. (2021). The role of additive manufacturing for biomedical applications: A critical review. Journal of Manufacturing Processes, 64(November 2020), 828–850. https://doi.org/10.1016/j.jmapro.2021.02.022
Kumar Shetty, S., Sundar Santhanakrishnan, S., Padurao, S., & Mirazkar Dasharatharao, P. (2024). Prioritizing Biomaterial Driven Clinical Bioactivity Over Designing Intricacy during Bioprinting of Trabecular Microarchitecture: A Clinician’s Perspective. ACS Omega. https://doi.org/10.1021
/acsomega.3c08112
Lee, J., Walker, J., Natarajan, S., & Yi, S. (2019). Prediction of geometric characteristics in polycaprolactone (PCL) scaffolds produced by extrusion-based additive manufacturing technique for tissue engineering. Rapid Prototyping Journal, 26(2), 238–248. https://doi.org/10.1108/RPJ-08-2018-0219
Lee, S.-H., Cho, Y. S., Hong, M. W., Lee, B.-K., Park, Y., Park, S.-H., Kim, Y. Y., & Cho, Y.-S. (2017). Mechanical properties and cell-culture characteristics of a polycaprolactone kagome-structure scaffold fabricated by a precision extruding deposition system. Biomedical Materials, 12(5), 055003. https://doi.org/10.1088/1748-605X/aa8357
Lefèvre, E., Farlay, D., Bala, Y., Subtil, F., Wolfram, U., Rizzo, S., Baron, C., Zysset, P., Pithioux, M., & Follet, H. (2019). Compositional and mechanical properties of growing cortical bone tissue: a study of the human fibula. Scientific Reports, 9(1), 17629. https://doi.org/10.1038/s41598-019-54016-1
Lequin, M., Colard, T., Colombo, A., Le Cabec, A., Remy, F., & Schuh, A. (2025). Investigating Development in Human Evolution: Specificities, Challenges, and Opportunities. Evolutionary Anthropology: Issues, News, and Reviews, 34(1). https://doi.org/10.1002/evan.70001
Li, Y., Yang, C., Zhao, H., Qu, S., Li, X., & Li, Y. (2014). New developments of ti-based alloys for biomedical applications. Materials, 7(3), 1709–1800. https://doi.org/10.3390/
ma7031709
Lin, K., Yu, K., & Li, N. (2024). Modeling and Mechanical Performance Analysis of Porous Gradient Bone Scaffolds. https://doi.org/10.21203/rs.3.rs-4647620/v1
Liu, J., & Yan, C. (2018). 3D Printing of Scaffolds for Tissue Engineering. 3D Printing. https://doi.org/10.5772/intechopen.
78145
Liu, L., Liu, C., Deng, C., Wang, X., Liu, X., Luo, M., Wang, S., & Liu, J. (2023). Design and performance analysis of 3D-printed stiffness gradient femoral scaffold. Journal of Orthopaedic Surgery and Research, 18(1), 120. https://doi.org/10.1186/
s13018-023-03612-z
Liu, Y., Li, A., Chen, B., & Li, Y. (2021). Research on the Impact Resistance of the Periodic Helicoidal Multilayer Bionic Structure Based on Osteon Microstructure. https://doi.org/10.21203/rs.3.rs-354443/v1
Lv, Y., Wang, B., Liu, G., Tang, Y., Lu, E., Xie, K., Lan, C., Liu, J., Qin, Z., & Wang, L. (2021). Metal Material, Properties and Design Methods of Porous Biomedical Scaffolds for Additive Manufacturing: A Review. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.641130
Lyu, Y., Zhao, H., Wen, X., Lin, L., Schlarb, A. K., & Shi, X. (2021a). Optimization of 3D printing parameters for high-performance biodegradable materials. Journal of Applied Polymer Science, 138(32), 1–13. https://doi.org/10.1002/
app.50782
Lyu, Y., Zhao, H., Wen, X., Lin, L., Schlarb, A. K., & Shi, X. (2021b). Optimization of 3D printing parameters for high-performance biodegradable materials. Journal of Applied Polymer Science, 138(32), 1–13. https://doi.org/10.1002/app.50782
M. Anne Katzenberg, & Shelley R. Saunders. (2008). Biological Anthropology of the Human Skeleton (M. A. Katzenberg & S. R. Saunders, Eds.). Wiley. https://doi.org/10.1002/9780470
245842
Madrid, A. P. M., Vrech, S. M., Sanchez, M. A., & Rodriguez, A. P. (2019). Advances in additive manufacturing for bone tissue engineering scaffolds. Materials Science and Engineering C, 100(March), 631–644. https://doi.org/10.1016/j.msec.
2019.03.037
Mamidi, N., Ijadi, F., & Norahan, M. H. (2024). Leveraging the Recent Advancements in GelMA Scaffolds for Bone Tissue Engineering: An Assessment of Challenges and Opportunities. Biomacromolecules, 25(4), 2075–2113. https://doi.org/
10.1021/acs.biomac.3c00279
Mandl, K., Carlson, K. S. D., Brönnimann, D., McCall, A., Grassberger, M., Teschler-Nicola, M., Weiss-Krejci, E., & Metscher, B. (2021). The diagnostic utility of microCT for assessing bioerosion in archaeological bone. https://doi.org/10.21203/rs.3.rs-995605/v1
Manoj, A., & Panda, R. C. (2022). Biodegradable Filament for 3D Printing Process: A Review. Engineered Science. https://doi.org/10.30919/es8d616
Martelli, N., Serrano, C., Van Den Brink, H., Pineau, J., Prognon, P., Borget, I., & El Batti, S. (2016). Advantages and disadvantages of 3-dimensional printing in surgery: A systematic review. Surgery (United States), 159(6), 1485–1500. https://doi.org/10.1016/j.surg.2015.12.017
Masood, S. H. (2014). Advances in Fused Deposition Modeling. In Comprehensive Materials Processing (Vol. 10). Elsevier. https://doi.org/10.1016/B978-0-08-096532-1.01002-5
Meng, D., Hou, Y., Kurniawan, D., Weng, R.-J., Chiang, W.-H., & Wang, W. (2024). 3D-Printed Graphene and Graphene Quantum Dot-Reinforced Polycaprolactone Scaffolds for Bone-Tissue Engineering. ACS Applied Nano Materials, 7(1), 1245–1256. https://doi.org/10.1021/acsanm.3c05225
Mohamed, O. A., Masood, S. H., & Bhowmik, J. L. (2015). Optimization of fused deposition modeling process parameters: a review of current research and future prospects. Advances in Manufacturing, 3(1), 42–53. https://doi.org/10.1007/s40436-014-0097-7
Mohd Ariffin, M. K. A., Sukindar, N. A., Hang Tuah Bin Baharudin, B. T., Jaafar, C. N. A. B., & Ismail, M. I. S. Bin. (2019). The effect of process parameters in extruding scaffold design using synthetic biomaterials. International Journal of Modern Manufacturing Technologies, 11(3 Special Issue), 9–20.
Montero, J., Becerro, A., Cristina, G., Pardal-pel, B., Quispe-l, N., & Blanco, J. (2021). Main 3D Manufacturing Techniques for Customized Bone. Materials, 1–24.
Montero, J., Becerro, A., Pardal-Peláez, B., Quispe-López, N., Blanco, J. F., & Gómez-Polo, C. (2021). Main 3D manufacturing techniques for customized bone substitutes. A systematic review. Materials, 14(10). https://doi.org/10.3390/ma14102524
Mota, R. C. de A. G., Silva, E. O. da, Lima, F. F. de, Menezes, L. R. de, & Thiele, A. C. S. (2016). 3D Printed Scaffolds as a New Perspective for Bone Tissue Regeneration: Literature Review. Materials Sciences and Applications, 07(08), 430–452. https://doi.org/10.4236/msa.2016.78039
Murab, S., Herold, S., Hawk, T., Snyder, A., Espinal, E., & Whitlock, P. (2023). Advances in additive manufacturing of polycaprolactone based scaffolds for bone regeneration. Journal of Materials Chemistry B, 11(31), 7250–7279. https://doi.org/10.1039/D2TB02052A
Naghieh, S., & Chen, X. (2021). Printability–A key issue in extrusion-based bioprinting. Journal of Pharmaceutical Analysis, 11(5), 564–579. https://doi.org/10.1016/
j.jpha.2021.02.001
Naghieh, S., Sarker, M. D., Sharma, N. K., Barhoumi, Z., & Chen, X. (2020). Printability of 3D printed hydrogel scaffolds: Influence of hydrogel composition and printing parameters. Applied Sciences (Switzerland), 10(1). https://doi.org/
10.3390/app10010292
Narayanan, L. K., & Shirwaiker, R. A. (2020). Experimental Characterization and Finite Element Modeling of the Effects of 3D Bioplotting Process Parameters on Structural and Tensile Properties of Polycaprolactone (PCL) Scaffolds. Applied Sciences, 10(15), 5289. https://doi.org/10.3390/app10155289
Nesic, D., Schaefer, B. M., Sun, Y., Saulacic, N., & Sailer, I. (2020). 3D Printing Approach in Dentistry: The Future for Personalized Oral Soft Tissue Regeneration. Journal of Clinical Medicine, 9(7), 2238. https://doi.org/10.3390/jcm9072238
Niinomi, M. (2008). Mechanical biocompatibilities of titanium alloys for biomedical applications. Journal of the Mechanical Behavior of Biomedical Materials, 1(1), 30–42. https://doi.org/10.1016/j.jmbbm.2007.07.001
Niza, E., Božik, M., Bravo, I., Clemente-Casares, P., Lara-Sanchez, A., Juan, A., Klouček, P., & Alonso-Moreno, C. (2020). PEI-coated PLA nanoparticles to enhance the antimicrobial activity of carvacrol. Food Chemistry, 328, 127131. https://doi.org/10.1016/j.foodchem.2020.127131
Nyberg, E., O’Sullivan, A., & Grayson, W. (2019). scafSLICR: A MATLAB-based slicing algorithm to enable 3D-printing of tissue engineering scaffolds with heterogeneous porous microarchitecture. PLOS ONE, 14(11), e0225007. https://doi.org/10.1371/journal.pone.0225007
Nyman, J. S., Makowski, A. J., Patil, C. A., Masui, T. P., O’Quinn, E. C., Bi, X., Guelcher, S. A., Nicollela, D. P., & Mahadevan-Jansen, A. (2011). Measuring Differences in Compositional Properties of Bone Tissue by Confocal Raman Spectroscopy. Calcified Tissue International, 89(2), 111–122. https://doi.org/10.1007/s00223-011-9497-x
O’Brien, C. M., Holmes, B., Faucett, S., & Zhang, L. G. (2015). Three-Dimensional Printing of Nanomaterial Scaffolds for Complex Tissue Regeneration. Tissue Engineering Part B: Reviews, 21(1), 103–114. https://doi.org/10.1089/
ten.teb.2014.0168
O’Brien, F. J., Harley, B. A., Waller, M. A., Yannas, I. V., Gibson, L. J., & Prendergast, P. J. (2007). The effect of pore size on permeability and cell attachment in collagen scaffolds for tissue engineering. Technology and Health Care, 15(1), 3–17. https://doi.org/10.3233/thc-2007-15102
Oftadeh, R., Perez-Viloria, M., Villa-Camacho, J. C., Vaziri, A., & Nazarian, A. (2015). Biomechanics and Mechanobiology of Trabecular Bone: A Review. In Journal of Biomechanical Engineering (Vol. 137, Number 1). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/
1.4029176
Okolie, O., Stachurek, I., Kandasubramanian, B., & Njuguna, J. (2020). 3D Printing for Hip Implant Applications: A Review. Polymers, 12(11), 2682. https://doi.org/10.3390/
polym12112682
Oladapo, B. I., Ismail, S. O., Adebiyi, A. V, Omigbodun, F. T., Olawumi, M. A., & Olawade, D. B. (2021a). Nanostructural interface and strength of polymer composite scaffolds applied to intervertebral bone. Colloids and Surfaces A, 627(February).
Oladapo, B. I., Ismail, S. O., Adebiyi, A. V, Omigbodun, F. T., Olawumi, M. A., & Olawade, D. B. (2021b). Nanostructural interface and strength of polymer composite scaffolds applied to intervertebral bone. Colloids and Surfaces A, 627(February).
Oleksy, M., Dynarowicz, K., & Aebisher, D. (2023). Rapid Prototyping Technologies: 3D Printing Applied in Medicine. Pharmaceutics, 15(8), 2169. https://doi.org/10.3390/
pharmaceutics15082169
O’Shea, T. M., & Miao, X. (2008). Bilayered Scaffolds for Osteochondral Tissue Engineering. Tissue Engineering Part B: Reviews, 14(4), 447–464. https://doi.org/10.1089/
ten.teb.2008.0327
Ou-Yang, Q., Guo, B., & Xu, J. (2018). Preparation and Characterization of Poly(butylene succinate)/Polylactide Blends for Fused Deposition Modeling 3D Printing. ACS Omega, 3(10), 14309–14317. https://doi.org/
10.1021/acsomega.8b02549
Özcan, M., Hotza, D., Fredel, M. C., Cruz, A., & Volpato, C. A. M. (2021). Materials and Manufacturing Techniques for Polymeric and Ceramic Scaffolds Used in Implant Dentistry. Journal of Composites Science, 5(3), 78. https://doi.org/10.3390/
jcs5030078
Pahlevanzadeh, F., Emadi, R., Valiani, A., Kharaziha, M., Poursamar, S. A., Bakhsheshi-Rad, H. R., Ismail, A. F., RamaKrishna, S., & Berto, F. (2020). Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends. Materials, 13(11), 2663. https://doi.org/10.3390/ma13112663
Paladini, F., & Pollini, M. (2022). Novel Approaches and Biomaterials for Bone Tissue Engineering: A Focus on Silk Fibroin. Materials, 15(19), 6952. https://doi.org/
10.3390/ma15196952
Parulski, C., Jennotte, O., Lechanteur, A., & Evrard, B. (2021). Challenges of fused deposition modeling 3D printing in pharmaceutical applications: Where are we now? Advanced Drug Delivery Reviews, 175. https://doi.org
/10.1016/j.addr.2021.05.020
Patel, P. K., Sonagara, C., Kumar, S., Meena, K., & Banerjee, S. S. (2025). 3D Printing of Bioplastics for Medical Applications. Polymer Engineering & Science, 65(8), 3880–3903. https://doi.org/10.1002/pen.27266
Pattanashetti, N. A., Biscaia, S., Moura, C., Mitchell, G. R., & Kariduraganavar, M. Y. (2019). Development of novel 3D scaffolds using BioExtruder by the incorporation of silica into polycaprolactone matrix for bone tissue engineering. Materials Today Communications, 21(September), 1–12. https://doi.org/10.1016/j.mtcomm.2019.100651
Pazhamannil, R. V., & Alkhedher, M. (2025). Advances in additive manufacturing for bone tissue engineering: materials, design strategies, and applications. Biomedical Materials, 20(1), 012002. https://doi.org/10.1088/1748-605X/ad9dce
Pecci, R., Baiguera, S., Ioppolo, P., Bedini, R., & Del Gaudio, C. (2020a). 3D printed scaffolds with random microarchitecture for bone tissue engineering applications: Manufacturing and characterization. Journal of the Mechanical Behavior of Biomedical Materials, 103(November 2019). https://doi.org/10.1016/j.jmbbm.2019.103583
Pecci, R., Baiguera, S., Ioppolo, P., Bedini, R., & Del Gaudio, C. (2020b). 3D printed scaffolds with random microarchitecture for bone tissue engineering applications: Manufacturing and characterization. Journal of the Mechanical Behavior of Biomedical Materials, 103(November 2019). https://doi.org/10.1016/j.jmbbm.2019.103583
Penumakala, P. K., Santo, J., & Thomas, A. (2020). A critical review on the fused deposition modeling of thermoplastic polymer composites. Composites Part B: Engineering, 201(May), 108336. https://doi.org/10.1016/j.compositesb.2020.108336
Perdomo-Pantoja, A., Holmes, C., Cottrill, E., Rindone, A. N., Ishida, W., Taylor, M., Tomberlin, C., Lo, S. L., Grayson, W. L., & Witham, T. F. (2021). Comparison of Freshly Isolated Adipose Tissue-derived Stromal Vascular Fraction and Bone Marrow Cells in a Posterolateral Lumbar Spinal Fusion Model. Spine, 46(10), 631–637. https://doi.org/10.1097/BRS.000
0000000003709
Pereira, G. G., Figueiredo, S., Fernandes, A. I., & Pinto, J. F. (2020). Polymer Selection for Hot-Melt Extrusion Coupled to Fused Deposition Modelling in Pharmaceutics. Pharmaceutics, 12(9), 795. https://doi.org/10.3390/pharmaceutics12090795
Peto, M., García-Ávila, J., Rodriguez, C. A., Siller, H. R., da Silva, J. V. L., & Ramírez-Cedillo, E. (2024). Review on structural optimization techniques for additively manufactured implantable medical devices. Frontiers in Mechanical Engineering, 10. https://doi.org/10.3389/fmech.2024.1353108
Pichetpan, K., Singsuwan, P., & Mahakkanukrauh, P. (2024). Age estimation using medial clavicle by histomorphometry method with artificial intelligence: A review. Medicine, Science and the Law, 64(4), 329–342. https://doi.org/10.1177/
00258024241270779
Pierantozzi, D., Scalzone, A., Jindal, S., Stīpniece, L., Šalma-Ancāne, K., Dalgarno, K., Gentile, P., & Mancuso, E. (2020). 3D printed Sr-containing composite scaffolds: Effect of structural design and material formulation towards new strategies for bone tissue engineering. Composites Science and Technology, 191. https://doi.org/10.1016/j.compscitech.
2020.108069
Popescu, D., Zapciu, A., Amza, C., Baciu, F., & Marinescu, R. (2018). FDM process parameters influence over the mechanical properties of polymer specimens: A review. Polymer Testing, 69(April), 157–166. https://doi.org/10.1016/j.polymertesting.2018.05.020
Prabhakar, M. M., Saravanan, A. K., Lenin, A. H., Leno, I. J., Mayandi, K., & Ramalingam, P. S. (2020). A short review on 3D printing methods, process parameters and materials. Materials Today: Proceedings, 45, 6108–6114. https://doi.org/10.1016/j.matpr.2020.10.225
Prasadh, S., & Wong, R. C. W. (2018). Unraveling the mechanical strength of biomaterials used as a bone scaffold in oral and maxillofacial defects. Oral Science International, 15(2), 48–55. https://doi.org/10.1016/S1348-8643(18)30005-3
Pu’ad, N. A. S. M., Abdul Haq, R. H., Mohd Noh, H., Abdullah, H. Z., Idris, M. I., & Lee, T. C. (2019). Review on the fabrication of fused deposition modelling (FDM) composite filament for biomedical applications. Materials Today: Proceedings, 29(November 2018), 228–232. https://doi.org/10.1016
/j.matpr.2020.05.535
Qin, Y., Qi, Q., Scott, P. J., & Jiang, X. (2019). Determination of optimal build orientation for additive manufacturing using Muirhead mean and prioritised average operators. Journal of Intelligent Manufacturing, 30(8), 3015–3034. https://doi.org/10.1007/s10845-019-01497-6
Qu, H. (2020). Additive manufacturing for bone tissue engineering scaffolds. Materials Today Communications, 24(November 2019), 101024. https://doi.org/10.1016/j.mtcomm.2020.
101024
Qu, M., Wang, C., Zhou, X., Libanori, A., Jiang, X., Xu, W., Zhu, S., Chen, Q., Sun, W., & Khademhosseini, A. (2021). Multi‐Dimensional Printing for Bone Tissue Engineering. Advanced Healthcare Materials, 10(11). https://doi.org/10.1002/
adhm.202001986
Raje, V., Palekar, S., Banella, S., & Patel, K. (2022). Tunable Drug Release from Fused Deposition Modelling (FDM) 3D-Printed Tablets Fabricated Using a Novel Extrudable Polymer. Pharmaceutics, 14(10), 2192. https://doi.org/10.3390/pharmaceutics14102192
Ranat, K., Phan, H., Ellythy, S., Kenter, M., & Akkouch, A. (2025). Advancements in Musculoskeletal Tissue Engineering: The Role of Melt Electrowriting in 3D-Printed Scaffold Fabrication. Journal of Functional Biomaterials, 16(5), 163. https://doi.org/10.3390/jfb16050163
Raquez, J. M., Habibi, Y., Murariu, M., & Dubois, P. (2013). Polylactide (PLA)-based nanocomposites. Progress in Polymer Science, 38(10–11), 1504–1542. https://doi.org/10.1016/j.progpolymsci.2013.05.014
Ratheesh, G., Venugopal, J. R., Chinappan, A., Ezhilarasu, H., Sadiq, A., & Ramakrishna, S. (2017). 3D Fabrication of Polymeric Scaffolds for Regenerative Therapy. ACS Biomaterials Science & Engineering, 3(7), 1175–1194. https://doi.org/10.1021/acsbiomaterials.6b00370
Ratnayake, J. T. B., Mucalo, M., & Dias, G. J. (2017). Substituted hydroxyapatites for bone regeneration: A review of current trends. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 105(5), 1285–1299. https://doi.org/10.1002/jbm.b.33651
Rayegani, F., & Onwubolu, G. C. (2014). Fused deposition modelling (fdm) process parameter prediction and optimization using group method for data handling (gmdh) and differential evolution (de). International Journal of Advanced Manufacturing Technology, 73(1–4), 509–519. https://doi.org/10.1007/s00170-014-5835-2
Renders, G. A. P., Mulder, L., van Ruijven, L. J., & van Eijden, T. M. G. J. (2007). Porosity of human mandibular condylar bone. Journal of Anatomy, 210(3), 239–248. https://doi.org/10.1111/j.1469-7580.2007.00693.x
Roi, A., Ardelean, L. C., Roi, C. I., Boia, E.-R., Boia, S., & Rusu, L.-C. (2019). Oral Bone Tissue Engineering: Advanced Biomaterials for Cell Adhesion, Proliferation and Differentiation. Materials, 12(14), 2296. https://doi.org/10.3390/ma12142296
Sahmani, S., Khandan, A., Esmaeili, S., Saber-Samandari, S., Ghadiri Nejad, M., & Aghdam, M. M. (2020a). Calcium phosphate-PLA scaffolds fabricated by fused deposition modeling technique for bone tissue applications: Fabrication, characterization and simulation. Ceramics International, 46(2), 2447–2456. https://doi.org/10.1016/j.ceramint.2019.09.238
Sahmani, S., Khandan, A., Esmaeili, S., Saber-Samandari, S., Ghadiri Nejad, M., & Aghdam, M. M. (2020b). Calcium phosphate-PLA scaffolds fabricated by fused deposition modeling technique for bone tissue applications: Fabrication, characterization and simulation. Ceramics International, 46(2), 2447–2456. https://doi.org/10.1016/j.ceramint.2019.09.238
Sakthi Balan, G., & Aravind Raj, S. (2025). Effect of additives on the performance of extruded polymer composites: A comprehensive review. Journal of Thermoplastic Composite Materials, 38(2), 810–853. https://doi.org/10.1177/08927057
241261314
Samadian, H., Khastar, H., Ehterami, A., & Salehi, M. (2021). Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study. Scientific Reports, 11(1), 13877. https://doi.org/10.1038/s41598-021-93367-6
Samarawickrama, K. G. (2018). A review on bone grafting, bone substitutes and bone tissue engineering. ACM International Conference Proceeding Series, (September), 244–251. https://doi.org/10.1145/3239438.3239457
Samykano, M. (2021a). Mechanical Property and Prediction Model for FDM-3D Printed Polylactic Acid (PLA). Arabian Journal for Science and Engineering, 46(8), 7875–7892. https://doi.org/10.1007/s13369-021-05617-4
Samykano, M. (2021b). Mechanical Property and Prediction Model for FDM-3D Printed Polylactic Acid (PLA). Arabian Journal for Science and Engineering, 46(8), 7875–7892. https://doi.org/10.1007/s13369-021-05617-4
Serra, T., Planell, J. A., & Navarro, M. (2013a). High-resolution PLA-based composite scaffolds via 3-D printing technology. Acta Biomaterialia, 9(3), 5521–5530. https://doi.org/10.1016/j.actbio.2012.10.041
Serra, T., Planell, J. A., & Navarro, M. (2013b). High-resolution PLA-based composite scaffolds via 3-D printing technology. Acta Biomaterialia, 9(3), 5521–5530. https://doi.org/10.1016/j.actbio.2012.10.041
Sfeir, C., Fang, P.-A., Jayaraman, T., Raman, A., Xiaoyuan, Z., & Beniash, E. (2014). Synthesis of bone-like nanocomposites using multiphosphorylated peptides. Acta Biomaterialia, 10(5), 2241–2249. https://doi.org/10.1016/j.actbio.2014.01.007
Sharma, S., Mishra, A., Jain, V., & Gupta, V. (2025). Investigating the Influence of Additive Manufacturing and Ultrasonic Coating Parameters on Biopolymeric Scaffold Performance Using Response Surface Methodology. Biopolymers, 116(1). https://doi.org/10.1002/bip.23629
Shi, H., Zhou, Z., Li, W., Fan, Y., Li, Z., & Wei, J. (2021). Hydroxyapatite based materials for bone tissue engineering: A brief and comprehensive introduction. Crystals, 11(2), 1–18. https://doi.org/10.3390/cryst11020149
Shor, L., Güçeri, S., Wen, X., Gandhi, M., & Sun, W. (2007). Fabrication of three-dimensional polycaprolactone/ hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro. Biomaterials, 28(35), 5291–5297. https://doi.org/10.1016/j.biomaterials.2007.08.018
Sodupe Ortega, E., Sanz-Garcia, A., Pernia-Espinoza, A., & Escobedo-Lucea, C. (2019). Efficient Fabrication of Polycaprolactone Scaffolds for Printing Hybrid Tissue-Engineered Constructs. Materials, 12(4), 613. https://doi.org/10.3390/ma12040613
Sohrabian, M., Vaseghi, M., Khaleghi, H., Dehrooyeh, S., & Kohan, M. S. A. (2021a). Structural Investigation of Delicate-Geometry Fused Deposition Modeling Additive Manufacturing Scaffolds: Experiment and Analytics. Journal of Materials Engineering and Performance, 30(9), 6529–6541. https://doi.org/10.1007/s11665-021-05894-y
Sohrabian, M., Vaseghi, M., Khaleghi, H., Dehrooyeh, S., & Kohan, M. S. A. (2021b). Structural Investigation of Delicate-Geometry Fused Deposition Modeling Additive Manufacturing Scaffolds: Experiment and Analytics. Journal of Materials Engineering and Performance, 30(9), 6529–6541. https://doi.org/10.1007/s11665-021-05894-y
Solomon, I. J., Sevvel, P., & Gunasekaran, J. (2020). A review on the various processing parameters in FDM. Materials Today: Proceedings, 37(Part 2), 509–514. https://doi.org/10.1016/j.matpr.2020.05.484
Sousa, H. C., Ruben, R. B., & Viana, J. C. (2024). On the Fused Deposition Modelling of Personalised Bio-Scaffolds: Materials, Design, and Manufacturing Aspects. Bioengineering, 11(8), 769. https://doi.org/10.3390/
bioengineering11080769
Sow, W. T., Wang, Y., Chen, L., Lai, Q., Chen, Q., Chen, L., Zhang, C., & Li, H. (2023). Freeze‐Casted Keratin Matrix as an Organic Binder to Integrate Hydroxyapatite and BMP2 for Enhanced Cranial Bone Regeneration (Adv. Healthcare Mater. 16/2023). Advanced Healthcare Materials, 12(16). https://doi.org/10.1002/adhm.202370081
Stafin, K., Śliwa, P., & Piątkowski, M. (2023). Towards Polycaprolactone-Based Scaffolds for Alveolar Bone Tissue Engineering: A Biomimetic Approach in a 3D Printing Technique. International Journal of Molecular Sciences, 24(22), 16180. https://doi.org/10.3390/ijms242216180
Staiger, M. P., Pietak, A. M., Huadmai, J., & Dias, G. (2006). Magnesium and its alloys as orthopedic biomaterials: A review. Biomaterials, 27(9), 1728–1734. https://doi.org/10.1016/
j.biomaterials.2005.10.003
Stockhausen, K. E., Qwamizadeh, M., Wölfel, E. M., Hemmatian, H., Fiedler, I. A. K., Flenner, S., Longo, E., Amling, M., Greving, I., Ritchie, R. O., Schmidt, F. N., & Busse, B. (2021). Collagen Fiber Orientation Is Coupled with Specific Nano-Compositional Patterns in Dark and Bright Osteons Modulating Their Biomechanical Properties. ACS Nano, 15(1), 455–467. https://doi.org/10.1021/acsnano.0c04786
Su, Q., Qiao, Y., Xiao, Y., Yang, S., Wu, H., Li, J., He, X., Hu, X., Yang, H., & Yong, X. (2023). Research progress of 3D printed poly (ether ether ketone) in the reconstruction of craniomaxillofacial bone defects. Frontiers in Bioengineering and Biotechnology, 11. https://doi.org/10.3389/
fbioe.2023.1259696
Sun, Z. P., Guo, Y. B., & Shim, V. P. W. (2021a). Characterisation and modeling of additively-manufactured polymeric hybrid lattice structures for energy absorption. International Journal of Mechanical Sciences, 191(August 2020). https://doi.org/10.1016/j.ijmecsci.2020.106101
Sun, Z. P., Guo, Y. B., & Shim, V. P. W. (2021b). Characterisation and modeling of additively-manufactured polymeric hybrid lattice structures for energy absorption. International Journal of Mechanical Sciences, 191(August 2020). https://doi.org/10.1016/j.ijmecsci.2020.106101
Szcześ, A., Hołysz, L., & Chibowski, E. (2017). Synthesis of hydroxyapatite for biomedical applications. Advances in Colloid and Interface Science, 249(April), 321–330. https://doi.org/10.1016/j.cis.2017.04.007
Takabatake, K., Yamachika, E., Tsujigiwa, H., Takeda, Y., Kimura, M., Takagi, S., Nagatsuka, H., & Iida, S. (2014). Effect of geometry and microstructure of honeycomb TCP scaffolds on bone regeneration. Journal of Biomedical Materials Research Part A, 102(9), 2952–2960. https://doi.org/
10.1002/jbm.a.34966
Tian, L., Zhang, Z., Tian, B., Zhang, X., & Wang, N. (2020). Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds. RSC Advances, 10(8), 4805–4816. https://doi.org/10.1039/C9RA10275B
Tollemar, V., Collier, Z. J., Mohammed, M. K., Lee, M. J., Ameer, G. A., & Reid, R. R. (2016). Stem cells, growth factors and scaffolds in craniofacial regenerative medicine. Genes & Diseases, 3(1), 56–71. https://doi.org/10.1016/
j.gendis.2015.09.004
Toosi, S., Javid-Naderi, M. J., Tamayol, A., Ebrahimzadeh, M. H., Yaghoubian, S., & Mousavi Shaegh, S. A. (2024). Additively manufactured porous scaffolds by design for treatment of bone defects. Frontiers in Bioengineering and Biotechnology, 11. https://doi.org/10.3389/fbioe.2023.1252636
Turnbull, G., Clarke, J., Picard, F., Riches, P., Jia, L., Han, F., Li, B., & Shu, W. (2018). 3D bioactive composite scaffolds for bone tissue engineering. Bioactive Materials, 3(3), 278–314. https://doi.org/10.1016/j.bioactmat.2017.10.001
van der Meijden, R. H. M., Daviran, D., Rutten, L., Walboomers, X. F., Macías-Sánchez, E., Sommerdijk, N., & Akiva, A. (2023). A 3D Cell-Free Bone Model Shows Collagen Mineralization is Driven and Controlled by the Matrix. Advanced Functional Materials, 33(42), 2212339. https://doi.org/https://doi.org/
10.1002/adfm.202212339
Venugopal, J., Vadgama, P., Kumar, T. S. S., & Ramakrishna, S. (2007). Biocomposite nanofibres and osteoblasts for bone tissue engineering. Nanotechnology, 18(5), 055101. https://doi.org/10.1088/0957-4484/18/5/055101
Wang, W., Zhang, B., Li, M., Li, J., Zhang, C., Han, Y., Wang, L., Wang, K., Zhou, C., Liu, L., Fan, Y., & Zhang, X. (2021). 3D printing of PLA/n-HA composite scaffolds with customized mechanical properties and biological functions for bone tissue engineering. Composites Part B: Engineering, 224(July), 109192. https://doi.org/10.1016/j.compositesb.2021.109192
Wang, Z., Sun, Y., & Li, C. (2024). Advances in 3D printing technology for preparing bone tissue engineering scaffolds from biodegradable materials. Frontiers in Bioengineering and Biotechnology, 12. https://doi.org/10.3389/fbioe.2024.1483547
Wang, Z., Xu, J., Zhu, J., Fang, H., Lei, W., Qu, X., Cheng, Y. Y., Li, X., Guan, Y., Wang, H., & Song, K. (2025). Osteochondral Tissue Engineering: Scaffold Materials, Fabrication Techniques and Applications. Biotechnology Journal, 20(1). https://doi.org/10.1002/biot.202400699
Whitebone, S. A., Bari, A. S. M. H., Gavrilova, M. L., & Anderson, J. S. (2021). A multimethod approach to the differentiation of enthesis bone microstructure based on soft tissue type. Journal of Morphology, 282(9), 1362–1373. https://doi.org/10.1002/jmor.21391
Winarso, R., Anggoro, P. W., Ismail, R., Jamari, J., & Bayuseno, A. P. (2022). Application of Fused Deposition Modeling (FDM) on Bone Scaffold Manufacturing Process: A Review. SSRN Electronic Journal, 8(April), e11701. https://doi.org/10.2139/ssrn.4103975
Winarso, R., Ismail, R., Anggoro, P. W., Jamari, J., & Bayuseno, A. P. (2023a). A scoping review of the additive manufacturing of mandibular implants. In Frontiers in Mechanical Engineering (Vol. 9). Frontiers Media S.A. https://doi.org/10.3389/
fmech.2023.1079887
Winarso, R., Ismail, R., Anggoro, P. W., Jamari, J., & Bayuseno, A. P. (2023b). Finite Element Analysis Of Irregular Porous Scaffold For Bone Tissue Engineering. ARPN Journal of Engineering and Applied Sciences, 18(6), 569–580. www.arpnjournals.com
Winarso, R., Ismail, R., Anggoro, P. W., Jamari, J., & Bayuseno, A. P. (2024a). Numerical study on mechanical properties of cubic porous bone scaffold: Effect of unit cell type. AIP Conference Proceedings, 3074(1). https://doi.org/10.1063/5.0211261
Winarso, R., Ismail, R., Anggoro, P. W., Jamari, J., & Bayuseno, A. P. (2024b). Porous Structures Simulation Analysis: The Effect of Different Strut Geometry on the Bone Scaffold. In Irwansyah, Mohd. Iqbal, S. Huzni, & Akhyar (Eds.), Proceedings of the 4th International Conference on Experimental and Computational Mechanics in Engineering (pp. 103–112). Springer Nature Singapore.
Wojnicz, W., Augustyniak, M., & Borzyszkowski, P. (2021a). Mathematical approach to design 3D scaffolds for the 3D printable bone implant. Biocybernetics and Biomedical Engineering, 41(2), 667–678. https://doi.org/10.1016/
j.bbe.2021.05.001
Wojnicz, W., Augustyniak, M., & Borzyszkowski, P. (2021b). Mathematical approach to design 3D scaffolds for the 3D printable bone implant. Biocybernetics and Biomedical Engineering, 41(2), 667–678. https://doi.org/10.1016/
j.bbe.2021.05.001
Wong, K. I., Zhong, Y., Li, D., Cheng, Z., Yu, Z., & Wei, M. (2021a). Modified porous microstructure for improving bone compatibility of poly-ether-ether-ketone. Journal of the Mechanical Behavior of Biomedical Materials, 120. https://doi.org/10.1016/j.jmbbm.2021.104541
Wong, K. I., Zhong, Y., Li, D., Cheng, Z., Yu, Z., & Wei, M. (2021b). Modified porous microstructure for improving bone compatibility of poly-ether-ether-ketone. Journal of the Mechanical Behavior of Biomedical Materials, 120. https://doi.org/10.1016/j.jmbbm.2021.104541
Wu, D., Spanou, A., Diez-Escudero, A., & Persson, C. (2020). 3D-printed PLA/HA composite structures as synthetic trabecular bone: A feasibility study using fused deposition modeling. Journal of the Mechanical Behavior of Biomedical Materials, 103(August 2019), 103608. https://doi.org/10.1016
/j.jmbbm.2019.103608
Xie, Z., Gao, M., Lobo, A. O., & Webster, T. J. (2020). 3D Bioprinting in Tissue Engineering for Medical Applications: The Classic and the Hybrid. Polymers, 12(8), 1717. https://doi.org/10.3390/polym12081717
Xu, N., Ye, X., Wei, D., Zhong, J., Chen, Y., Xu, G., & He, D. (2014). 3D artificial bones for bone repair prepared by computed tomography-guided fused deposition modeling for bone repair. ACS Applied Materials and Interfaces, 6(17), 14952–14963. https://doi.org/10.1021/am502716t
Xu, Z., Omar, A. M., & Bartolo, P. (2021). Experimental and Numerical Simulations of 3D-Printed Polycaprolactone Scaffolds for Bone Tissue Engineering Applications. Materials, 14(13), 3546. https://doi.org/10.3390/ma14133546
Yan, Y., Sun, S., Pillay, S., & Ning, H. (2025). Printability and mechanical behaviors of continuous polyamide fiber reinforced polycaprolactone composites. Polymer Composites, 46(5), 4491–4505. https://doi.org/10.1002/pc.29254
Yang, Y., Wang, G., Liang, H., Gao, C., Peng, S., Shen, L., & Shuai, C. (2019). Additive manufacturing of bone scaffolds. International Journal of Bioprinting, 5(1), 1–25. https://doi.org/10.18063/IJB.v5i1.148
Yousefi, A.-M., James, P. F., Akbarzadeh, R., Subramanian, A., Flavin, C., & Oudadesse, H. (2016). Prospect of Stem Cells in Bone Tissue Engineering: A Review. Stem Cells International, 2016(1). https://doi.org/10.1155/2016/6180487
Yuan, Z., Li, F., Zhang, P., Chen, B., & Xue, F. (2014). Mechanical properties study of particles reinforced aluminum matrix composites by micro-indentation experiments. Chinese Journal of Aeronautics, 27(2), 397–406. https://doi.org/10.1016/j
.cja.2014.02.010
Yusop, A. H., Bakir, A. A., Shaharom, N. A., Abdul Kadir, M. R., & Hermawan, H. (2012). Porous Biodegradable Metals for Hard Tissue Scaffolds: A Review. International Journal of Biomaterials, 2012, 1–10.
https://doi.org/10.1155/2012/641430
Zafar, M. J., Zhu, D., & Zhang, Z. (2019). 3D Printing of Bioceramics for Bone Tissue Engineering. Materials, 12(20), 3361. https://doi.org/10.3390/ma12203361
Zhang, B., Wang, L., Song, P., Pei, X., Sun, H., Wu, L., Zhou, C., Wang, K., Fan, Y., & Zhang, X. (2021). 3D printed bone tissue regenerative PLA/HA scaffolds with comprehensive performance optimizations. Materials and Design, 201, 109490. https://doi.org/10.1016/j.matdes.2021.109490
Zhang, H., Mao, X., Zhao, D., Jiang, W., Du, Z., Li, Q., Jiang, C., & Han, D. (2017). Three dimensional printed polylactic acid-hydroxyapatite composite scaffolds for prefabricating vascularized tissue engineered bone: An in vivo bioreactor model. Scientific Reports, 7(1), 15255. https://doi.org/10.1038/s41598-017-14923-7
Zhang, Q., Wu, W., Qian, C., Xiao, W., Zhu, H., Guo, J., Meng, Z., Zhu, J., Ge, Z., & Cui, W. (2019). Advanced biomaterials for repairing and reconstruction of mandibular defects. Materials Science and Engineering C, 103. https://doi.org/
10.1016/j.msec.2019.109858
Zhang, Y., Wu, D., Zhao, X., Pakvasa, M., Tucker, A. B., Luo, H., Qin, K. H., Hu, D. A., Wang, E. J., Li, A. J., Zhang, M., Mao, Y., Sabharwal, M., He, F., Niu, C., Wang, H., Huang, L., Shi, D., Liu, Q., … El Dafrawy, M. (2020). Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.598607
Zheng, C., & Zhang, M. (2023). 3D-printed PCL/β-TCP/CS composite artificial bone and histocompatibility study. Journal of Orthopaedic Surgery and Research, 18(1), 981. https://doi.org/10.1186/s13018-023-04489-8
Zhou, Y.-H., Guo, Y., Zhu, J.-Y., Tang, C.-Y., Zhao, Y.-Q., & Zhou, H.-D. (2022). Spheroid co-culture of BMSCs with osteocytes yields ring-shaped bone-like tissue that enhances alveolar bone regeneration. Scientific Reports, 12(1), 14636. https://doi.org/10.1038/s41598-022-18675-x
